规格：50ug/100ug
货号：PD7878
价格：50ug/1150元，100ug/1900元
| 货号 | PD7878 |
|---|---|
| 品牌 | Pinofi |
| 描述 | Anti-SARS-CoV-2 RBD Antibody (1A12) [1A12] (VK565103) is a human monoclonal antibody detecting RBD in ELISA, IHC, WB. Highlights
|
| 种属反应性 | SARS-CoV-2 (2019-nCoV) |
| 应用 | ELISA, IHC, WB,IF |
| 宿主 | Human |
| 同种型 | IgG1, kappa |
| 克隆号 | 1A12 |
| 克隆类型 | Monoclonal |
| 靶标 | Spike glycoprotein, S glycoprotein, E2, Peplomer protein, Spike protein S1, S, Receptor-Binding Domain, RBD |
| 内毒素水平 | Please contact with the lab for this information. |
| 纯度 | >95% as determined by SDS-PAGE. |
| 纯化方式 | Protein A/G purified from cell culture supernatant. |
| Accession号 | P0DTC2 |
| 状态 | Liquid |
| 保存溶液 | 0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA. |
| 产品使用信息 | 应用方法稀释比例ELISA1:2000-1:20000WB1:500-1:2000 |
| 稳定性和存储 | Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term. |
| 背景 | Spike glycoprotein (RBD) is a ~141 kDa protein. Attaches the virion to the cell membrane by interacting with host receptor, initiating the infection. The major receptor is host ACE2. When S2/S2' has been cleaved, binding to the receptor triggers direct fusion at the cell membrane. When S2/S2' has not been cleaved, binding to the receptor results in internalization of the virus by endocytosis using host TFRC and GRM2 and leading to fusion of the virion membrane with the host endosomal membrane. Alternatively, may use NRP1/NRP2 and integrin as entry receptors. 1. Hoffmann, M. et al. (2020) Cell 181, 271-280.e8. PMID: 32142651 2. Walls, AC. et al. (2020) Cell 181, 281-292.e6. PMID: 32155444 3. Cao, W. et al. (2021) Biophysical journal 120, 1011-1019. PMID: 33607086 4. Takeda, M. (2022) Microbiology and immunology 66, 15-23. PMID: 34561887 5. Wrapp, D. et al. (2020) Science (New York, N.Y.) 367, 1260-1263. PMID: 32075877 6. Ou, X. et al. (2020) Nature communications 11, 1620. PMID: 32221306 7. Wang, J. et al. (2021) Cell discovery 7, 119. PMID: 34903715 8. Wang, X. et al. (2023) Journal of virology 97, e0161122. PMID: 36779763 9. Daly, JL. et al. (2020) Science (New York, N.Y.) 370, 861-865. PMID: 33082294 10. Cantuti-Castelvetri, L. et al. (2020) Science (New York, N.Y.) 370, 856-860. PMID: 33082293 |
| Note | For research use only. |
